A Theoretical Study of CCD4a Dioxygenase of Citrus, a Cleavage

Transcrição

Artigo Geral 38
A Theoretical Study of CCD4a
Dioxygenase of Citrus, a Cleavage
Enzyme of Carotenoids in Plants
Mauricio Vega-Teijido, Margot P. Zunini, Carolina López & Maria J. Rodrigo
FOHDYHG 6WLOO UHPDLQV XQFOHDU WKH VSHFL¿F SRVLWLRQ ZKHUH
Introduction
%
!
"
#
$
"
%
&
%
'
&
"
(
'
)
+
(
*
'
)
*
,
'
!
+
,
*
$
'
!
"
*
-
!
&
/
0
-
/
/
!
.
]HD[DQWKLQ DQG ȕFDURWHQH DV VXEVWUDWHV ,Q WKH FDVH RI
&&'DRIFLWUXVWKHVSHFL¿FSRVLWLRQLVQRWFRQ¿UPHG\HW
>@7KHÀRZHUVZLWKZKLWHSHWDOVVKRZHGDKLJKH[SUHVVLRQ
'
SHUFHSWLRQ RI TXDOLW\ $PRQJ WKHP &&'E LV WKH ¿UVW
&
(
#
)
(
*
'
)
*
SURGXFWV DUH ȕFLWUDXULQ RU ȕDSRFDURWHQDO GHSHQGLQJ
ȕFDURWHQH UHVSHFWLYHO\ ȕFU\SWR[DQWKLQ VXEVWUDWH FDQ
)LJXUH ;
D
B
:
9
=
A
D
2
7
1
2
;
3
=
4
>
5
2
6
C
4
7
6
8
E
2
9
6
F
4
4
:
9
4
;
4
<
7
6
7
8
=
<
=
>
?
4
6
@
6
<
7
3
8
<
6
<
A
B
=
7
3
:
=
;
;
8
H
!
D
C
I
J
4
?
A
4
K
L
M
N
O
A
8
P
Q
=
O
;
:
4
2
8
6
C
R
S
T
T
T
1
U
V
W
B
C
4
G
X
4
E
8
;
7
6
Y
9
=
2
4
;
;
=
;
V
D
Z
5
8
2
=
;
[
&
Artigo Geral 38
Methods
$
]
^
"
/
\
/
_
0
`
"
!
&
/
'
$
b
&
]
!
a
b
_
\
[
'
!
,
'
0
!
[
0
+
!
'

"
!
,
'
!
'
c

.
`
&
$
$6(VFRULQJSRVLQJDQ$PEHUUH¿QHPHQWDQG/RQGRQ'*
UHVFRULQJ7KHIRUFH¿HOGXVHGLQ0'ZDV$PEHUZLWKD
'
RUGHU WR ¿QG DOO WKH SRVVLEOH SRVHV LQ WKH DFWLYH VLWH7KH
ȕFU\SWR[DQWKLQ DQG ȕFDURWHQH UHVSHFWLYHO\ )LJXUH VLJQL¿FDQFH
Results and Discussion
0
'
$
'
_
!
#
0
0
'
,
!
'
c
)LJXUH 5
=
B
[
"
d
e
'
)
$
-
,
f
$
!
'
'
D
9
C
4
4
6

=
2
7
8
2
8
2
E
F
6
D
<
A
:
4
8
=
@
;
4

=
F
4
-
$
f
"
2

4
<
4
2
A
=
5
=
=
C
8
9
4
<
A
2
8
D
7
<
6
C
3
4
7
4
8
A
6
=
D
2
<
7
;
9
8
8
<
8
7
E
4
4
F
7
;
=
3
>
8
7
3
7
3
4

5
=
8
4
g
h
7
i

=
j
D
7
=
>
7
3
4
6
2
7
8
E
4
;
8
i
k
l
m
n
ȕ
k
l
o
p
4
2
6
7
4
6
C

G
;
8

W
;
J
3

8
;
6
;
8
G
7

4
z
v
x

~

o
v
o
v
r
s
l
t
j
i
k
l
m
n
ȕ
k
i
o
o
y
u
x
o

7DEOH/RQGRQ'*VFRUHVIRUWKHEHVW¿YHVROXWLRQV,Qȕ
D
5
<
7

v
=
;
=
C
D
7
8
=
<
;
2
=
9
9
4
;
:
=
<
A
7
=
7
3
4
2
=
<
>
=
9
5
6
7
8
=

2
<
9

:

8
7
7
=
@
3
7
6
3
<
7
4
3
8

o
=
8
4
7

=
D
7
=
>
7
3
4
g
6
2
7
8
E
4
;
8
7
4
7
:
4
8
9
4
A
l
h
k
h
q
u
~
x

u
~
u
~
o
|
l
x

x
z

x
z
v
x

x

}
o
y
x

{
o
u

o
y
x

w
o
w
x

h
i
j
i
k
l
m
n
ȕ
k
l
p
r
s
l
t
j
i
k
l
m
n
q
k
ȕ
i
o
t
p
u
u
u
t
o
v
w
x
y
o
y
x
y
l
h
k
o
v
w
z
|
l
v
o
y
o
v
w
x
u
x
o
y
v
w
x

y

~

u
~
x
z
u
~
u
~
o
x
o
~
z
o
y
x

|
{
u
o
v
w
x

x
o
y
x

u
~

o
y
o

l

o
x
z
/
-
&

x
v

\
u
x

/
`
"
}
o
u
y

x
w
z

y
~
o
t
o
u
z
~

z
}
{
u
x
x
u
h
~
v
w
y
~
q
u
v
o
~
u
G
o
[
6
D
<
!
2

_
5

2
t
p
~
u
x
u
C
=
G
u
=
4
;

2
<
8
u
7
>

v
=
K
G
t
4

u
u
7
4
q
u
o
8

u
=
<
¿QDOVWUXFWXUHRIWKHOLJDQGVKRZHGDQLVRPHUL]DWLRQZKHQ
A
;
7DEOH/RQGRQ'*VFRUHVIRUWKHEHVW¿YH=LVRPHUVVROXWLRQV,Q
ȕFU\SWR[DQWKLQFROXPQRQHVROXWLRQFRUUHVSRQGVWRWKHFRQIRUPDWLRQ
6

2

4
0
C
&

u
o

x

H
X
4
E
8
;
7
6
Y
9
=
2
4
;
;
=
;
V
D
Z
5
8
2
=
;
O
A
8
P
Q
=
O
;
:
4
2
8
6
C
R
S
T
T
T
1
U
V
W
D
C
I
J
4
?
A
4
K
L
M
N
SRVVLEOH RULHQWDWLRQV RI ȕFU\SWR[DQWKLQ LQ WKH DFWLYH
Acknowledgments
]
e
e
#
^
_
d
#
_
a
#
#
#
^
e
%
_
%

References
M
G

3
1
5
D
8
5
8

6
7
=

G
5
=

8

;
G
3

8

5

=

7
=

Y
1
C
G
6
<

7
8
Y
<
A
3

6
;

8
X
=
G
C
G

M

=

;
K

3
8
M
=
M

¡
6
¢

£
1
M
G
K

6
L
<
M
A
G
H
K
G
X
D
W
B
9
8
6
=
:

4

9
=
=

9
6

F
=
6
?

=

;
G

X

G
6

*yPH]*yPH]/
¢
5
B
3
9
C
6
6
?

4
G
5

G

G
4

9

<
9
¤
?
<
¤
A
4
4
?
?

A
J
4
G

¥
6
9
9
6
5
4
<
4
C
C
<

G

H

¦

U
8
=
C

3
4
5
K
§
¢

¢
¨

©
K

§
M
¨
£
K

§
K
N
G
5RGULJR0-$OTXp]DU%$OyV(0HGLQD9&DUPRQD
G
H

G
O

G
N

4
6
@
6
<
7
3
8
<
C
8
F
6
<
A
8
<
7
3
4
6
2
7
8
E
4
;
8
7
4
=
>

J

6
9
:

G
U
@
C
1
)LJXUH

4
=
2

8
9
4
J
G

4
4
G
2
6

<
G
2
O
=
5

<

<
9
4
8
D
7
Y
6
G
¢

G
C

4
C
=
D
§
C

U
X
L
=

2
C
<
B
9
C
G
K

1
G

C
¡
:

¨

8
¨
1

8

¬
6
6
6
3
¨
D
U
7
2
K

M

6

U
6
©
B
2

8
6
9

C
8
Z
¨

6
M
1
;

£
G

G

U

¬
4

§

ª
«

=
D
9
<
6
C
=
>
G
4
9

Y
G

1
2
3
D
C
?

¥
G
O
G

G
4
9
6
7
8
<
F
O
<
E
8
9
=
<
5
4
<
7

O

G
L
¡

G

3
4
5
8
C

=
5
:
D
7
8
<
F
¥
9
=
D
:

T
<
2
G

=
<
7
9
4
6
C

V
D
4
B
4
2

6
<
6
A
6
G
H
¨
7DEOH
>
=
9

J

E
2
4
6
C
2
9
6
D
F
C
6
4
7
E
8
=
6
<
C
;
i
j
i
D
4
;
>
=
9
7
3
4

6
B

2
6
C
I
5
=
C

6
<
k
l
m
n
A
r
s
l
t
j
i
k
l
m
n
k
t
{
9
A
A
4
E
8
6
7
8
=

Y
G
G

U
D
F
F

W
G

G
J
G

ª
®

9
2
3
G
U
8
=
2
3
4
5
G
U
8
=
:
3

;
G
N
N

x
w
{
z
x
v
|
l
}
w
x

{
z
x

s
l
t
j
i
k
l
m
n
k
o
p
o
p
w
x
w
{

x
w
q

}
i
t
l
h
k
h
o

x

{
z
x

q
-
-
$
f
_
'
_
/
$
$

^
$
)
-
,
f
$

G
<
Mauricio Vega-Teijidoa,b,
Margot Paulino Zuninia,
Carolina Lópeza & Maria J.
Rodrigoc
RWKHU SDUDPHWHUV LQÀXHQFLQJ WKH VHOHFWLYH FOHDYDJH RU
<
M
}
r
o
6
=
M
u
u
A
;
M
}
q
<
8

~
v
o
6
9
}
l
p
7
9
N
$YHUDJH6WG'HY
u
o
;
k
o
ȕ
ȕ
ȕ
6
L
G
u
h

M
8DE
g
G
\
FODUL¿HGLQQH[WVWHSVRIRXUZRUN
¯
Conclusions

8

=
8
U
<
>
¥

=

J
J
O
O
W
W
O
O

6
6
2
D
2
C
D
7
C
6
7
6
A
A
A
A
4
4
V
V
D
D
Z
Z
5
8
5
8
2
6
2
6

A
A
4
C
4
6
C
6
X
X
±

6
B
=
9
6
7
=
9
8
=

8
;
8
=
C
=
F
Z
6

U
8
=
7
4
2
<
4
<
=
C
=
F
Z
6
Y
=
;
7
2
=
;
4
2
3
6
T
<
;
7
8
7
D
7
=
A
F
9
=
²
D
Z
5
8
2
6

W
4
2
<
=
C
=
F
Z
6
A
4

C
8
5
7
=
;
T

W

1
T

S
6
C
4
<
2
8
6

O
;
:
6
!
C
I
J
4
?
A
4
K
L
M
N
³
6
G
H
D
4

U

7KHVH ¿QGLQJV ZLOO EH GLVFXVVHG FRQVLGHULQJ WKH
4
°
O
A
8
P
Q
=
O
;
:
4
2
8
6
C
R
S
T
T
T
1
U
V
W
X
4
E
8
;
7
6
Y
9
=
2
4
;
;
=
;
V
D
Z
5
8
2
=
;
[
`

A Theoretical Study of CCD4a Dioxygenase of Citrus, a Cleavage

Transcrição

Documentos relacionados

21_1465Atencio proof.indd