Curriculum ingles 2013

1. PERSONAL INFORMATION:
Name: Andrea Cristina Paula Lima
ID: 22513214-3 (identity foreigners permanently residing in Chile).
Date and place of birth: Rio de Janeiro - Brazil, 20/11/1977.
Nationality: Brazilian
Marital status: Married
Work address: Sergio Livingstone Pohlhammer St., n. 943, Independencia, Santiago de Chile,
Chile.
Work phone: 56-2-97861761, 56-2-9786603
Address: Francisco de Villagra, nº 6082, La Reina, Santiago de Chile, Chile.
Telephone: 56-2-6323641 / cel. 56-9-77071027
FAX: (56-2) 7776916.
E-mail: [email protected]
Workplace: Laboratory of Cellular and Molecular Biology, University of Chile, Santiago,
Chile.
Current position: Assistant Professor. Full time (44 hours)
2. ACADEMIC INFORMATION:
Basic Studies and Media
Technical School of Chemistry Federal do Rio de Janeiro, Rio de Janeiro, Brazil
Titles:
Professional Title: Pharmacist. Faculty of Pharmacy, Universidade Federal do Rio de Janeiro,
Rio de Janeiro, Brazil. (1998-2003).
Professional Title: Biotechnology Technician. Technical School of Chemistry Federal do Rio
de Janeiro, Rio de Janeiro, Brazil. (1992-1996).
Degree
PhD in Biological Chemistry. Institute of Medical Biochemistry, Universidade Federal do Rio
de Janeiro, Rio de Janeiro, Brazil. Thesis title: "Mechanisms of neuroprotection against the
toxicity induced by aggregates of β-amyloid peptide and possible implications to Alzheimer´s
disease treatment. (2003-2007).
Monographs:
MONOGRAPH: Techniques for bacteriological diagnosis by clinical examination. Author:
Andréa Paula Cristina Lima. Monograph for obtaining technical degree in Biotechnology.
(1996).
DOCTORAL THESIS: Mechanisms of neuroprotection against the toxicity of aggregated βamyloid peptide and possible implications for the treatment of Alzheimer's disease. (2007).
Distinctions obtained
Awards:
• 2013: Junior Faculty Award at the 11 th International Conference on Alzheimer's and
Parkinson's Diseases (AD / PD), Florence, Italy. For the exceptional professional contribution to
the study of Alzheimer's disease.
• 2012: Selected as an affiliate member of TWAS (The World Academy of Sciences) for the
period 2012-2017. Since 2007, TWAS annually elects excellence scientists younger than 40
years to be named Young Members for a period of five years. To be elected, Members of
TWAS Young must live and work in a developing country and have at least 10 international
publications. In the field of neuroscience, have been chosen as the sole representative of Chile
among the five elected Latin American researchers in 2012.
• 2011: Selected to participate in the 2012 Frontiers in Science Program of the Chilean
Academy of Sciences. This contest is intended to recognize excellence and potential of young
scientists in different areas of science and linked to the activities of the Academy.
• 2011: Selected to participate in the 61st Lindau Nobel Laureate Meeting dedicated to
Physiology / Medicine. Qualified in global competition among young researchers, Council for
the Lindau Nobel Laureate Meetings / Foundation Lindau at Lake Constance Nobelprizewinners
Meetings, Lindau, Germany.
• 2011: Junior Faculty Award at the 10th International Conference on Alzheimer's and
Parkinson's Diseases (AD / PD), Barcelona, Spain. For the exceptional professional contribution
to the study of Alzheimer's disease.
• 2010: Society for Neurosciences Chapter Postdoctoral Trainee Travel Award. Presentation of
the paper entitled "Aß Oligomers Stimulate Ca2 + release by RyR and Prevent RyR-mediated
spine remodeling induced by BDNF, in the" 40th Neuroscience Meeting "held in San Diego,
CA, USA.
• 2010: Insertion in the Program for Attraction and Integration of Advanced Human Capital
of the National Council of Scientific and Technological Research (CONICYT). Project
entitled "Renewing Academic Development of Molecular Medicine". This project aims at the
inclusion of excellent researchers to strengthen the scientific capacity of national academic
institutions, promoting the employment of highly qualified professionals in the academy. It is
obtained through a national competition of Attraction and Integration Program Advanced
Human Capital National Council of Scientific and Technological Research (CONICYT), Chile
(2010-2013).
• 2009: Conesul Young Scientist Award. Presentation of the talk entitled "Changes in ryanodine
receptor expression and activity in hippocampal neurons exposed to BDNF or beta-amyloid
peptide oligomers", at the Symposium "SBBq Cone Sul" in the XXXVIII Annual Meeting of
the Brazilian Society of Biochemistry and Molecular Biology (Sociedade Brasileira of
Biochemistry and Molecular Biology - SBBq) in Águas de Lindóia - São Paulo - Brazil (1619/05/2009).
• 2006: Distinction of Merit awarded by the Federação of Societies for Experimental Biology
(FESBE) for poster presentation entitled "Taurine accumulation of extracellular glutamate
blocks and Prevents neurotoxicity induced by β-amyloid in hippocampal and cortical neurons"
in the XXI Annual Meeting of the Federação of Societies for Experimental Biology (FESBE),
off Lindóia - São Paulo - Brazil (23-26/08/2006).
• 2005: Scholarship Note 10 issued by the National Foundation for Research Support of Rio de
Janeiro (Fundação de Amparo à Pesquisa Nacional do Rio de Janeiro - FAPERJ) for carrying
out the second stage of the doctoral thesis (03/08 / 2005-13/08/2007). This is a scholarship
specifically granted to outstanding doctoral students, who perform with excellence in the first
two years of thesis, as assessed by the resume and record notes Doctoral bouquets. (2005-2007)
• 2003: Qualified first in the entrance examination to the PhD in Biological Chemistry,
Institute of Medical Biochemistry, Universidade Federal do Rio de Janeiro, Brazil.
• 2002: HHMI Travel Fellowship. Grant from The Howard Hughes Medical Institute (HHMI)
to participate in the course entitled "Fluorescence Imaging in Neuroscience" held at the Center
for Scientific Studies, Valdivia, Chile (2002).
• 2002: IBRO Travel Fellowship. Grant from the International Brain Organization (IBRO) to
participate in the course entitled "Calcium signals and Neurotransmission" held at the Centre for
Cellular and Molecular Neuroscience of Valparaiso, Valparaiso University, Valparaiso, Chile
(2002).
Fellowships:
• Postdoctoral Fellowship granted by FONDECYT for the project entitled "Role of ryanodine
receptors on neuronal calcium signals and synaptic plasticity Alterations induced by the betaamyloid peptide", developed at the Laboratory for Calcium signals mediated by the Center for
Molecular Studies of the Cell, University of Chile, under the patronage of Dr. Cecilia Hidalgo.
(02/11/2007-07/03/2010).
• Doctoral Fellowship awarded by the National Research Council of Brazil (Conselho
Nacional de Pesquisa - CNPq) for carrying out the first stage of the doctoral thesis entitled
"Mechanisms to toxicidade neuroproteção against aggregates do peptídio β-amyloid and
possíveis implicações tratamento not Doença Alzheimer da "(03/08/2003-03/08/2005).
• Note 10 Scholarship granted by the Fundação de Amparo à Pesquisa do Rio de Janeiro
(FAPERJ) for carrying out the second stage of the doctoral thesis entitled "Mechanisms to
toxicidade neuroproteção against aggregates do peptídio β-amyloid and possíveis implicações
tratamento not given Alzheimer Doença "(03/08/2003-03/08/2005).
• Scientific Initiation Scholarship awarded by the National Research Council of Brazil
(Conselho Nacional de Pesquisa - CNPq) for the development of undergraduate work entitled
"Caracterização and its pharmacological modulação neurotoxicidade do peptídio beta amyloid"
(2001-2003).
• Scientific Initiation Scholarship awarded by the National Research Council of Brazil
(Conselho Nacional de Pesquisa - CNPq) for the development of undergraduate work entitled
"Effect of antifungal inhibitors of sphingolipid biosynthesis in Trypanosoma cruzi in axenic
culture." ( 1999-2000).
• Scientific Initiation Scholarship granted by the Oswaldo Cruz Foundation (Fundação
Oswaldo Cruz - FIOCRUZ-RJ) for conducting a further stay in the Laboratory of Clinical
Bacteriology Hospital Evandro Chagas (FIOCRUZ). Requirement for professional qualification
in Biotechnology Technician (03/1996-12/1996).
Scientific Societies:
•
•
•
•
•
Society for Neurosciences
Sociedade Brasileira de Neurociêncas e Comportamento
Neurotoxicity Society
Sociedad de Biología Celular de Chile
Sociedad de Biología de Chile
Grants:
•
Fondecyt Initiation “Unraveling the calcium signaling pathways that mediate the
neuronal dysfunctions induced by amyloid-beta peptide oligomers.” 2011-2014.
Principal Investigador.
•
U-networks Domeyko 2. Fondequip “BioMed-HPC: Red de Biología y Medicina
Computacional de Alto Rendimiento”. 2012. Co-investigator.
•
Fondequip “Cellomics: Plataforma automatizada de microscopia de alta complejidad
a grande escala con potencial biológico”. 2012. Co-investigator.
•
Strategic grants BNI (Biomedical Neuroscience Institute) titulado “Estudio de patrones
oscilatorios anormales en sujetos con enfermedad de Alzheimer incipiente y en
roedores inyectados con oligómeros del péptido beta-amiloide.” 2012-2013. Coinvestigador.
•
Insertion in the Program for Attraction and Integration of Advanced Human Capital
of the National Council of Scientific and Technological Research (CONICYT).
Project entitled "Renewing Academic Development of Molecular Medicine". This
project aims at the inclusion of excellent researchers to strengthen the scientific capacity
of national academic institutions, promoting the employment of highly qualified
professionals in the academy. It is obtained through a national competition of Attraction
and Integration Program Advanced Human Capital National Council of Scientific and
Technological Research (CONICYT), Chile (2010-2013). Co-investigator.
•
Postdoctoral Fellowship granted by FONDECYT for the project entitled "Role of
ryanodine receptors on neuronal calcium signals and synaptic plasticity Alterations
induced by the beta-amyloid peptide", developed at the Laboratory for Calcium signals
mediated by the Center for Molecular Studies of the Cell, University of Chile, under the
patronage of Dr. Cecilia Hidalgo. (02/11/2007-07/03/2010). Principal Investigator.
•
“Defining the role of the Unfolded Protein Response in Alzheimer s Disease”,
financiado por The Alzheimer´s Association, USA, 2011-2013. Co-investigator.
•
PhD granst for development of thesis Fundação Nacional de Amparo à Pesquisa do
Rio de Janeiro – FAPERJ.
Development Activities:
English Course for teachers of dental school coordinated by the Department of Education of
the Faculty of Dentistry, Universidad de Chile. (03-12/13).
Diplomat in University Teaching Based on Competencies coordinated by the Department of
Undergraduate belonging to the Vice Presidency of Academic Affairs at the University of Chile,
Santiago, Chile (09/2011-06/2012). The courses were "Basis for Curricular Modernization",
"Designing Training Programme", "Evaluation of Higher Education" and "Reflection on
Teaching practices".
Workshop “Building multiple choice questions” by the Department of Teacher Empowerment,
DECSA, Faculty of Medicine, University of Chile, Santiago, Chile (11/2011, 01/2012).
Workshop “Teacher Induction” for Scholars of Dentistry conducted by the Network for
Teaching Excellence and the Faculty of Dentistry, University of Chile, Santiago, Chile. (28/102/12/2011)
Course "Calcium signals and Neurotransmission," held at the Centre for Cellular and
Molecular Neuroscience of Valparaiso, Valparaiso University, Valparaiso, Chile, sponsored by
the International Brain Organization (IBRO) (18-25/11/2002).
Course "Fluorescence Imaging in Neuroscience" held at the Centre for Scientific Studies,
Valdivia, Chile, organized by "The U.S. National Academy of Sciences" and sponsored by The
Howard Hughes Medical Institute (HHMI) (29/11-08/12 / 2002).
Course "Scientific methodology for the study of Glycoconjugates in Microorganisms", Sixth
Week of Microbiology and Immunology, Institute of Microbiology Prof. Paulo de Goes,
Universidade Federal do Rio de Janeiro (28/09-02/10/1998).
Laboratory Assistance in the laboratory of Biotechnology, Federal Technical School of
Chemistry of Rio de Janeiro to support practical work in the fields of Technical Analysis in
Biochemistry, Animal Cell Culture, Plant Cell Culture and Molecular Biology for race
Biotechnology.
Languages:
Portuguese - written: native, reading: native; understanding: native.
English - written: advanced; reading: advanced; understanding: advanced.
Spanish - written: advanced; reading: advanced; understanding: advanced.
Tutoring:
• Victor Hugo Cornejo Corona (PhD). PhD candidate in the PhD Program of Biomedical
Sciences. Thesis entitled "Participation of the transcription factor XBP1 in UBQLN1 control
and its impact on the accumulation of Aß peptide in experimental models of Alzheimer's disease
(AD). Co-advisor. 2012-present.
• Pedro Lobos (Research Unit). Chemistry and Pharmacy student (fourth year), Faculty of
Chemical and Pharmaceutical Sciences, University of Chile. Title: The oligomers of the βamyloid peptide interferes with intracellular signaling of BDNF. 09/12-1/13.
• Barbara Bruna (Research Unit), Biochemistry Student (fourth year), Faculty of Chemical and
Pharmaceutical Sciences, University of Chile. Title: The oligomers of β-amyloid peptide
interferes with intracellular signaling of BDNF. 09/12-1/13.
• Thomas Perez-Luco (Research Unit). Medical Student (second year), Faculty of Medicine,
University of Chile. Title: Detection of isoform 2 ryanodine receptor (RyR2) in an experimental
model of Alzheimer's disease. 03-12/11/12.
• Joaquin Plaza (Research Unit). Medical Student (second year), Faculty of Medicine,
University of Chile. Title: Detection of isoform 2 ryanodine receptor (RyR2) in an experimental
model of Alzheimer's disease. 03-12/11/12.
• Macarena Las Heras (Research Unit). Biochemistry student (fifth year), Faculty of Chemical
and Pharmaceutical Sciences, University of Chile. Title: Relationship between the generation of
reactive oxygen species and calcium signals mediated by the ryanodine receptor in hippocampal
neurons exposed to oligomers of beta-amyloid peptide.03-09/11/12.
• Pablo Baratini (Research Unit). Candidate of Biochemistry Master Program, Faculty of
Chemical and Pharmaceutical Sciences, University of Chile. Title: Impact of the antioxidant Nacetylcysteine (NAC) on the changes in the expression of the ryanodine receptor caused by
AβOs 03-09/11/12.
• Vivian Motta de Almeida Ribeiro. Student of the Faculty of Medicine, Federal University of
Rio de Janeiro. Title: Mechanisms of neuroprotection against amyloid beta-toxicity (20062007).
• Jordano Brito-Moreira. Student of the Faculty of Pharmacy, Federal University of Rio de
Janeiro Universidade Federal do Rio de Janeiro Title: Mechanisms of neuroprotection against
amyloid beta-toxicity (2004-2007).
Research:
• 2011 – present. Conducting scientific research as Assistant Professor in the Department of
Basic Sciences, Faculty of Dentistry, University of Chile.
•
• 2010 – 2011 - Performing scientific research in the Institute of Biomedical Sciences,
Faculty of Medicine, University of Chile as a postdoctoral researcher at the Academy inserted
by Attraction Program and Integration of Advanced Human Capital National Council of
Scientific and Technological Research (CONICYT) in agreement with the Center for Molecular
Studies of the Cell, Institute of Biomedical Sciences, University of Chile.
• 2007-2010 - Completion of postdoctoral work at the Laboratory of Signal Mediated Calcium
- Center for Molecular Studies of the Cell - Institute of Biomedical Sciences, University of
Chile, developing scientific research postdoctoral fellowship granted by FONDECYT for
implementation of the project entitled "Role of ryanodine receptors on neuronal calcium signals
and synaptic plasticity Alterations induced by the beta-amyloid peptide."
• 2003-2007 - PhD theses in the Neurodegenerative Diseases Laboratory - Institute of Medical
Biochemistry - Federal University of Rio de Janeiro, developing scientific research Scholarship
Note 10 issued by the National Foundation for Research Support of Rio de Janeiro (FAPERJ)
(03/08/2003-13/08/2005) and PhD scholarship granted by the National Research Council of
Brazil (Conselho Nacional de Pesquisa - CNPq) (03/08/2005-13/08/2007) for the realization of
the doctoral thesis entitled "Mechanisms of neuroprotection against the toxicity of aggregated βamyloid peptide and possible implications for the treatment of Alzheimer's disease."
• 2000-2003 - Scientific Initiation in the Biophysics Laboratory Protein Chemistry - Institute
of Medical Biochemistry - Universidade Federal do Rio de Janeiro, developing scientific
research Scientific Initiation Scholarship awarded by the National Research Council of Brazil
(Conselho National Research - CNPq) for the development of undergraduate work entitled
"Characterization and pharmacological modulation of the neurotoxicity of beta-amyloid
peptide" (2001-2003).
• 1998-2000: - Scientific Initiation in the Laboratory of Surface Glycobiology - Institute of
Microbiology Professor Paulo de Goes - Universidade Federal do Rio de Janeiro, developing
scientific research Scientific Initiation Scholarship awarded by the National Council for
Scientific Research Brazil (Conselho Nacional de Pesquisa - CNPq) for the development of
undergraduate work entitled "Partial purification of an O-Gluco-N-transferase Acetilgalactosil
Trypanosoma cruzi" (1998-1999) and Effect of anti fungal biosynthesis inhibitors sphingolipids
in Trypanosoma cruzi in axenic culture. "(1999-2000).
Book chapters:
1. The hormonal GH/IGF-1 axis, the physical activity and the ageing: Benefits and cliffs
given by supplementation. Andréa Paula Cristina Lima, Paulo Cesar de Carvalho Alves,
Luciano Dias Ferrão Max. Biochemistry ergogenic two resources. Shape Editor. Rio de Janeiro.
2007. ISBN 85-85253-81-9. 2010.
2. Clinical, genetic and biochemical basis of the pathophysiology and treatment of
Parkinson´s disease. Paulo R. Louzada and Andréa C. Paula-Lima. Molecular Basis em Clinic:
Um Estudo explanatory. Part VII: MOLECULAR BASIS two disturbances OJ E PERIFERICO
central nervous system. Editors: Paulo Cesar de Carvalho Alves, Andrea da Poian and
Agessandro Abrahão. 2010.
Publications in international journals
1. Dysregulation of glutamatergic neurotransmission in Alzheimer's disease (in preparation)
Paula-Lima, A.C., Brito-Moreira, J., Ferreira, S.T. Review.
2. Amyloid β-peptide oligomers decrease non-transferrin-bound iron uptake and do not
modify iron toxicity in primary hippocampal neurons. (2012) San Martin, C.D., Paula-Lima,
A.C., Adasme, T., Hidalgo, C. BioMetals. 25(4)805-13.
3. The antioxidant N-Acetyl-Cysteine prevents the mitochondrial fragmentation induced by
soluble Amyloid-β peptide oligomers. (2012) San Martin, C.D., Adasme, T., Hidalgo, C., PaulaLima, A.C. Neurodegenerative Diseases. 10(1-4):34-7.
4. Differential gene expression in adult human brain slices treated with Alzheimer's toxic
amyloid-β oligomers. (2011) Sebollela A., Freitas-Correa, L., Oliveira FF Paula-Lima AC, L.
Cardoso, C. Torres, Leon S., Marcondes J., Carraro, DM, Brentani H., De Felice, F . G., Sergio
T. Ferreira. Journal of Biological Chemistry. 287 (10) :7436-45.
5. Amyloid β peptide oligomers Stimulate RyR-mediated Ca2 + release inducing mitochondrial
fragmentation in hippocampal neurons and Prevent RyR-mediated dendritic spine remodeling
produced by BDNF-mediated Prevent ryanodine receptors induced spine growth in
hippocampal neurons by BDNF. (2011) Paula-Lima, BC, Adasme, T. San Martin, C., Sebollela,
A., Hetz, C., Carrasco, MA, Ferreira, ST, C. Hidalgo. Antioxidant and Redox Signaling. 4 (7)
:1209-23.
6. Involvement of ryanodine receptors in neurotrophin-induced hippocampal synaptic plasticity
and spatial memory formation. (2011) Adasme T. *, P. Haeger *, Paula-Lima AC, Espinoza, I.,
Carrasco MA, Hidalgo C. * These authors contributed equally to the work. Proc Natl Acad Sci
U S A. 108 (7) :3029-34.
7. Aß Oligomers Induce Glutamate Release from Hippocampal Neurons. (2011) Brito-Moreira,
J. *, Paula-Lima AC *., Bomfim TR, Oliveira FF, Sepulveda, FJ, De Mello, FG, Aguayo, LG,
Panizzutti, RA, Ferreira ST #. * These authors contributed equally to this work. # These authors
divide the work dese last authorship. Current Alzheimer's Research. 8 (5) :552-62.
8. Human apolipoprotein AI binds amyloid-β and Prevents Aß-induced neurotoxicity. (2009)
Paula-Lima AC *, Tricerri MA *, J. Brito-Moreira, Bomfim TR, FF Oliveira, MH Magdesian,
Grinberg LT, Panizzutti RA, Ferreira ST * These authors contributed equally to this work. Int J
Biochem Cell Biol 41 (6) :1361-70.
9. Small-molecule inhibitors reduce excess amyloid aggregation in a trisomy 16 mouse cortical
cell line. (2008) Paula-Lima AC, Arriagada C., Cardenas AM, Toro R., R. Caviedes, ST
Ferreira, P. Caviedes. Biological Research. 41: 129-136.
10. Activation of GABAA receptors by taurine and muscimol blocks the neurotoxicity of betaamyloid in hippocampal and cortical neurons. (2005) Paula-Lima AC, De Felice FG, BritoMoreira J, Ferreira ST. Neuropharmacology. 49: 1140-1148.
11. Taurine Prevents the neurotoxicity of beta-amyloid and glutamate receptor agonists:
activation of GABA receptors and possible Implications for Alzheimer's disease and other
neurological disorders. (2004) Louzada PR, AC Paula-Lima, DL Mendonca-Silva, Noel FF, de
Mello FG, Ferreira ST. The FASEB Journal. 18: 511-518.
12. Neuroprotection against Aß toxicity and glutamate: GABA receptors are Involved? (2003)
Paula-Lima AC, Louzada PR, de Mello FG, Ferreira ST. Neurotoxicity Research. 5:323-327
13. Dual role of glutamatergic neurotransmission on amyloid beta 1-42 aggregation and
neurotoxicity in embryonic avian retina. (2001) Louzada PR, Paula Lima AC, de Mello FG,
Ferreira ST. Neuroscience Letters. 301:59-63.
Abstracts in Scientific Meetings:
1. ROS and calcium in Alzheimer’s disease. Andrea Paula-Lima, Facultad de Odontologia,
Universidad de Chile. Presentación oral en el Simposio Role of reactive oxygen species
in the physiology and pathophysiology of the CNS en el I Congreso FALAN (Federación
de Asociaciones Latinoamericanas y del Caribe de Neurociencias). Cancún, México. 49 de Noviembre 2012.
2. Unraveling the Ca2+ signaling pathways that mediate the neuronal dysfunctions induced
by amyloid-β peptide oligomers. Paula-Lima, A.C.1, San Martin2, C., Baratini, P.2,
Perez-Luco, T. 2, Plaza, J. 2, Adasme T.2 and Hidalgo C.2. 1Faculty of Dentistry, 2Faculty
of Medicine, Universidad de Chile. Presentación oral en el Minisymposium IV “Cell
Physyology” en la Sociedad de Biología Celular de Chile. LV Reunión Anual de la
Sociedad de Biología de Chile. Puerto Varas, Chile. 23-27 de Octubre 2012.
3. Synaptic plasticity induced by BDNF requires ROS and RyR-mediated Ca2+ signals.
$Adasme T, $Figueroa P, $Bruna B, #Paula-Lima AC, $,§Hidalgo C. $CEMC, BNI &
§ICBM, F. Medicina, #F. Odontología, Universidad de Chile. LV Reunión Anual de la
Sociedad de Biología de Chile. Puerto Varas, Chile. 23-27 de Octubre 2012.
4. Role of mitochondria in neuronal Ca2+ and ROS signals induced by the Alzheimer´s
disease-associated amyloid-β peptide oligomers. 1SanMartín CD, 1,2Hidalgo C,
1,3Paula-Lima AC. 1CEMC, BNI and 2ICBM; Facultad de Medicina; 3Facultad de
Odontología. LV Reunión Anual de la Sociedad de Biología de Chile. Puerto Varas,
Chile. 23-27 de Octubre 2012.
5. The ER stress factor XBP1 controls secretion of amyloid-b in experimental models of
Alzheimer’s Disease. Víctor Hugo Cornejo1,2, Peter Thielen1,2, René Vidal1,2,
Claudia Molina1,2, Gabriela Martinez1,2, Andrea Paula-Lima1,2, and Claudio
Hetz1,2,3. 1Biomedical Neuroscience Institute, 2Center for Molecular Studies of the
Cell, Faculty of Medicine, Universidad de Chile and 3Neurounion Biomedical
Foundation, Santiago, Chile. LV Reunión Anual de la Sociedad de Biología de Chile.
Puerto Varas, Chile. 23-27 de Octubre 2012.
6. Calcium and ROS jointly contribute to the synaptic plasticity defects induced by amyloid-β
peptide oligomers, the distinctive markers of Alzheimer's disease. Paula-Lima, A. C.,
Hidalgo, C. Presentación oral. XII Conferencia & XXII Reunión General de la
TWAS (The World Academy of Sciences), Tianjin, China, 17-22 Septiembre 2012.
7. Amyloid
β-peptide oligomers stimulate RyR-mediated Ca2+ release inducing
mitochondrial fragmentation in hippocampal neurons and prevent RyR-mediated dendritic
spine remodeling produced by BDNF. Paula-Lima, A. C., Adasme, T., San Martín, C.,
Sebollela, A., Hetz, C., Carrasco, M.A., Ferreira, S.T., Hidalgo, C. Puerto Varas. 6-9 de
noviembre de 2011. Presentación oral del trabajo de incorporación a la Sociedad de
Biología Celular de Chile. LIV Reunión Anual de la Sociedad de Biología de Chile.
8. Iron and amyloid β peptide oligomers enhance ryanodine receptor-mediated calcium
release in primary hippocampal neurons which in turn stimulates mitocondrial fission.
Hidalgo, C., SanMartin, C., Paula-Lima, A.C., Hartel S., Garcia, A., Nuñez, M.T. “8th
IBRO Congress in Neuroscience”, realizado en Florence, Italy (14/07 a 18/07/11).
9. Aβ oligomers induce glutamate release from hippocampal neurons. Brito-Moreira, J.,
Paula-Lima, A.C., Bomfim, T.R., Oliveira, F.F., Sepulveda, F.J., De Mello, F.G.,
Aguayo, L.G., Panizzutti, R., Ferreira, S.T. “23rd Biennial Joint Meeting of the
International Society for Neurochemistry (ISN) and the European Society for
Neurochemistry (ESN)”, realizado en Athens, Greece (28/08 a 01/09 2011).
10. Amyloid beta peptide oligomers impair calcium-mediated dendritic spine remodeling.
Paula-Lima, A.C., Adasme, T., San Martin, C.D., Hidalgo C. “Bayer Science &
Education Foundation”, presentación oral realizada en Mainau Island, Germany (26/0601/07/2011).
11. Amyloid beta peptide oligomers stimulate sustained Ca2+ release mediated by ryanodine
receptors in hippocampal neurons, prompting mitocondrial fragmentation and preventing
BDNF-induced spine remodeling. Paula-Lima, A.C., Adasme, T., SanMartín, C.,
Härtel, S., Carrasco, M.A. and Hidalgo, C. Presentación oral. “10th International
Conference on Alzheimer and Parkinson (ADPD)”, realizado en Barcelona, Spain
(ADPD 2011).
12. Aβ oligomers prevent Ca2+-­‐‑mediated dendritic spine remodeling. Paula-­‐‑Lima A.C. Presentación oral. Symposium Mechanisms of Neuroplasticity: A view from an aged synapse. Centro Interdisciplinario de Neurociencias de Valparaíso, Valparaiso, Chile, International Brain Organization (26/11/2010).
13. Aβ oligomers stimulate Ca2+ release from intracellular stores via ryanodine receptors
(RyR) and prevent RyR-mediated dendritic spine remodeling promoted by BDNF in
hippocampal neurons. Paula-Lima A.C., Adasme T., SanMartín, C., Sebollela A.,
Hetz., C., Carrasco M.A., Ferreira S.T., Hidalgo C. Annals of the 39nd Society for
Neuroscience Annual Meeting San Diego, EUA. (13 a 17/11/2010).
14. Aβ oligomers stimulate Ca2+ release from intracellular stores via ryanodine receptors
(RyR) and prevent RyR-mediated dendritic spine remodeling promoted by BDNF in
hippocampal neurons. Paula-Lima A.C., Adasme T., SanMartín, C., Sebollela A.,
Hetz., C., Carrasco M.A., Ferreira S.T., Hidalgo C. Travel Award Recipients Poster
Session. Society for Neuroscience 11th Annual Chapter Awards Reception and Social,
San Diego, California, EUA. 39nd Society for Neuroscience Annual Meeting
(13/11/2010).
15. Iron and Amyloid β Peptide Oligomers Stimulate Mitochondrial Fission in Primary
Hippocampal Neurons. SanMartín, C.D., Paula-Lima, A.C., Härtel, S., García, A.,
Núñez, M.T., Hidalgo, C. Annals of the 39nd Society for Neuroscience Annual Meeting,
San Diego, EUA. (13 a 17/11/2010).
16. Aβ Oligomers Instigate the Release of excitatory amino acids from Hippocampal
Neurons. Brito-Moreira, J., Paula-Lima A.C., Bomfim T.R., Oliveira F.F., Sepúlveda,
F.J., De Mello, F.G., Aguayo, L.G., Panizzutti, R.A, Ferreira S.T. Annals of the
Alzheimer´s Association International Conference, Hawaii, EUA. (10 a 15/07/2010).
17. Iron and Amyloid β Peptide Oligomers Stimulate Mitochondrial Fission in Primary Hippocampal Neurons by Enhancing Ryanodine Receptor-­‐‑Mediated Calcium Release. San Martín, C.D., Paula-­‐‑Lima, A.C., Härtel, S., García, A., Núñez, M.T., Hidalgo, C. LIII Reunión Anual Sociedad de Biología de Chile (30 /09/2010). 18. Aβ Oligomers Instigate the Release of excitatory amino acids from HippocampalჼNeurons. Brito-­‐‑Moreira, J., Paula-­‐‑Lima A.C., Bomfim T.R., Oliveira F.F., Sepúlveda, F.J., De Mello, F.G., Aguayo, L.G., Panizzutti, R.A, Ferreira S.T. The Alzheimer´s Association International Conference, Hawaii, EUA. (10 a 15/07/2010).
19. La estimulación eléctrica de campo modula la expresión de la isoforma 2 del receptor
de ryanodina (RyR2) en neuronas de hipocampo en cultivo primario. Espinoza, I.,
Riquelme, D., Adasme, T., Paula-Lima, A.C., Leal, N., Hidalgo, C., Carrasco, M.A.
LII Sociedad Chilena de Biología, Pucón, Chile. (17-20/11/2009).
20. Amyloid-β oligomers prevent ryanodine receptor-mediated spine morphology changes
induced by brain derived neurotrophic factor. Adasme T., Paula-Lima A.C., Sebollela
A., Fernandez P., Ferreira S.T., Carrasco M.A., Hidalgo C. (2009). LII Sociedad
Chilena de Biología, Pucón, Chile. (17-20/11/2009).
21. Aβ oligomers and fibrils increase extracellular excitatory amino acids in cultured
hippocampal neurons. Brito-Moreira, J., Paula-Lima, A.C., Bomfim, T.R., Oliveira,
F.F., Sepulveda, F.J., De Mello, F.G., Aguayo, L.G., Panizzutti, R., Ferreira, S.T. XXIV
Annual Meeting of the Federation of Experimental Biology Societies (FESBE), Águas
de Lindóia, SP, Brasil, (19/08 a 22/08/2009).
22. Iron and amyloid β oligomers stimulate ryanodine receptor-mediated calcium release
and mitochondrial fission in hippocampal neurons. SanMartín, C.D., Paula-Lima,
A.C., Eisner, V., Härtel, S., García, A, Muñoz, P. Núñez, M.T., Hidalgo, C. V Reunión
Annual da Sociedad Chilena de Neurociencias, Coquimbo, Chile. (23 a 25/09/2009).
23. Changes in ryanodine receptor expression and activity in hippocampal neurons
exposed to BDNF or amyloid β peptide oligomers. Paula-Lima A.C., Adasme T.,
Sebollela A., Humeres A., Muñoz P., Olivero P., Leal N., Ferreira S.T., Carrasco M.A.,
Hidalgo C. SBBq-Conesul Young Scientist Symposia during the XXXVIII Annual
Meeting of the Brazilian Society of Biochemistry and Molecular Biology, Águas de
Lindóia, SP, Brazil (16 a 19/05/2009).
24. Changes in ryanodine receptor expression and activity in hippocampal neurons
exposed to BDNF or amyloid β peptide oligomers. Adasme T., Paula-Lima A.C.,
Humeres A., Muñoz P., Olivero P., Leal N., Ferreira S.T., Carrasco M.A., Hidalgo C.
Annals of the 37nd Society for Neuroscience Annual Meeting, Washington, DC, EUA.
(15 a 19/11/2008).
25. Function and expression of ryanodine receptors in hippocampal neurons exposed to βamyloid peptide aggregates. Paula-Lima A.C., Adasme T., Humeres A., Sebollela A.,
Hetz C., Carrasco M.A., Ferreira S.T., Hidalgo C. Annals of the I Congress
IBRO/LARC of Neurosciences for Latin America, Caribbean and Iberian Peninsula,
Buzios, RJ, Brazil. (01 a 04/11/2008).
26. Oligomers and fibrils of the β-amyloid peptide increase extracellular excitatory amino
acids in hippocampal cultures. Brito-Moreira, J., Paula-Lima, A.C., Oliveira, F.F.,
Bomfim, T.R., De Mello F.G., Panizzutti, R.A., Ferreira, S.T. Annals of the I Congress
IBRO/LARC of Neurosciences for Latin America, Caribbean and Iberian Peninsula,
Buzios, RJ, Brazil. (01 a 04/11/2008).
27. Involvement and interplay of glutamate and GABAA receptors in the toxicity of the betaamyloid peptide. Paula-Lima, A.C., Brito-Moreira, J., Almeida, V., Panizzutti, R.A.,
Ferreira, S.T. Annals of the XXII Reunión Annual de la Sociedad Chilena de Biología
Celular, Pucon, Chile. (07-11/10/2007).
28. Taurine blocks accumulation of extracellular glutamate and prevents neurotoxicity
induced by β-amyloid in hippocampal and cortical neurons. Paula-Lima, A.C., BritoMoreira, J. Panizzutti, R.A., De Felice, F.G., de Mello, F.G., Ferreira, S.T. Annals of
the XXI Annual Meeting of the Federation of Experimental Biology Societies, Águas de
Lindóia, SP, Brazil (23-26/08/2006).
29. Human apolipoprotein A-I interacts with beta-amiloide (Aβ) and protects cortical and
hippocampal neurons against Aβ-induced toxicity. Brito-Moreira, J., Paula-Lima, A.
C., Tricerri, M.A., Ferreira, S.T. Annals of the XXI Annual Meeting of the Federation
of Experimental Biology Societies, Águas de Lindóia, SP, Brazil (23-26/08/2006).
30. Human apolipoprotein A-I interacts with beta-amiloide (Aβ) and protects cortical and
hippocampal neurons against Aβ-induced toxicity. Brito-Moreira, J., Paula-Lima, A.
C., Tricerri, M.A. e Ferreira, S.T. Annals of the XXXV Annual Meeting of the
Brazilian Society of Biochemistry and Molecular Biology, Águas de Lindóia, SP, Brazil
(01-04/07/2006).
31. Paula-Lima, A.C. The amyloid cascade and the anti-Abeta treatment (conferencia) y
participación en mesa redonda titulada “New therapeutic approaches in the treatment of
Alzheimer´s disease”. Presentación oral XXIII Brazilian Congress of Psychiatry, Belo
Horizonte, MG, Brazil (10/2005).
32. Activation of GABA A receptors by taurine and muscimol blocks the neurotoxicity of
beta-amyloid in hippocampal and cortical neurons. Paula-Lima, A.C., Brito-Moreira,
J., Panizzutti, R.A., De Felice, F.G., Ferreira, S.T. Annals of the XXXIV Annual Meeting
of the Brazilian Society of Biochemistry and Molecular Biology, Águas de Lindóia, SP,
Brazil (02 a 05/07/2005).
33. Activation of GABA receptors prevents the neurotoxicity of beta-amyloid in rat cortical
neurons. Paula-Lima, A.C, Brito-Moreira, J., De Felice, F.G., de Mello, F.G., Ferreira,
S.T. Presentación oral Annals of the XXXIII Annual Meeting of the Brazilian Society of
Biochemistry and Molecular Biology, Caxambú, MG, Brazil (15 a 18/05/2004).
34. Stability of honeycomb beta-amyloid aggregates investigated by light microscopy and
light scattering in comparison with general mie scattering theory. Holub O., PaulaLima A.C., Ferreira S.T. Annals of the XXXIII Annual Meeting of the Brazilian Society
of Biochemistry and Molecular Biology, Caxambú, MG, Brazil. (15 a 18/05/2004).
35. Large aggregates of the amyloid-β peptide 1-42: Honeycomb formation and stability
investigated by light microscopy and light scattering in comparison with general Mie
scattering theory. A potential in vitro model system for amyloid plaques? Holub, O.,
Paula-Lima A.C., Ferreira S.T. "I Latin American Protein Society (LAPS) Meeting",
realizado en Angra dos Reis, RJ, Brazil (8 a 12/11/ 2004).
36. Inhibitors of amyloidogenesis decrease intracellular amyloid accumulation in a cortical
cell line derived from trisomy 16 mouse. Paula-Lima A.C., Arriagada C., Cardenas
A.M., Toro R., Caviedes R., Caviedes P., Ferreira S.T. Annals of the First Neuroscience
Symposium of the International Institute for Neuroscience of Natal, Natal, RN, Brazil.
(03 a 07/04/2004).
37. Novel anti-amyloidogenic compounds decrease intracellular accumulation of amyloid
in a cortical cell line derived from trisomy 16 mouse. Paula-Lima A.C., Arriagada C.,
Cardenas A.M., Toro R., Caviedes R., Caviedes P., Ferreira S.T. Annals of the XXXII
Annual Meeting of the Brazilian Society of Biochemistry and Molecular Biology,
Caxambú, MG, Brazil. (17-20/05/2003).
38. Nitrophenols reduce the content if intracellular amyloid in the CTb neuronal cell line,
derived from the trisomy 16 mouse. Paula-Lima A.C., Arriagada C., Cardenas A.M.,
Toro R., Caviedes R., Caviedes P., Ferreira S.T. Annals of the 32nd Society for
Neuroscience Annual Meeting, Washington, DC, E.U.A. (02-07/11/2002).
39. On the role of eletrostatic interactions in beta-amyloid stability. Louzada, P.R., PaulaLima A.C., De Felice F.G. e Ferreira S.T.. Annals of the XXXI Annual Meeting of the
Brazilian Society of Biochemistry and Molecular Biology, Caxambú, MG, Brasil. (1821/05/2002).
40. Taurine protects neurons in culture against beta-amyloid neurotoxicity by activation of
gabaergic transmission. Louzada, P.R., Paula-Lima, A.C., Mendonça-Silva, D.L.,
Noel, F.F., de Mello, F.G. and Ferreira S.T. Annals of the XXXI Annual Meeting of the
Brazilian Society of Biochemistry and Molecular Biology, Caxambú, MG, Brazil. (1821/05/2002).
41. Contributions of eletrostatic interactions to the stability of amyloid fibrils formed by
amylin and beta-amyloid. Lopes, D.H., Louzada, P.R., Paula-Lima A.C., De Felice
F.G. e Ferreira S.T. Annals of the XXX Annual Meeting of the Brazilian Society of
Biochemistry and Molecular Biology, Caxambú, MG, Brazil. (19-22/05/2001).
42. Role of glutamatergic and gabaergic transmission on beta-amyloid neurotoxicity.
Possible implications for alzheimers disease therapeutics. Louzada P.R., Paula-Lima
A.C., Mendonça-Silva D.L., Noel F.N., de Mello F.G., Ferreira S.T. Arquivos de
Neuro-psiquiatria - Jornal Oficial da Academia Brasileira de Neurologia. III Reunião
de Pesquisadores em Doença de Alzheimer e Desordens Relacionadas, Rio de Janeiro,
RJ, Brazil (24/11/2001).
43. Role of glutamatergic neurotransmission on amyloid beta 1-42 aggregation and
neurotoxicity in embryonic avian retina. Paula-Lima A.C., Louzada P.R., MendonçaSilva D.L., Noel F.F., de Mello F.G., Ferreira S.T. Annals of the XXX Annual Meeting
of the Brazilian Society of Biochemistry and Molecular Biology, Caxambú, MG, Brazil.
(19-22/05/2001).
44. Inhibitors of sphingolipid biosynthesis and their effects on trypanosoma cruzi growth in
an axenic culture. Paula-Lima A.C., Santiago A.P., Mendonça-Previato L., Previato
J.O. Annals of the XXIX Annual Meeting of the Brazilian Society of Biochemistry and
Molecular Biology realizado en Caxambú, MG, Brazil. (27 a 30/05/2000).
45. Ether lipids X ceramides in GPI anchors of trypanosomatids: first round. Heise, N.,
Paula-Lima A.C., Hart, D.T., Mendonça-Previato L. and Previato J.O., Heise N.
Annals of the XXVI Annual Meeting on Basic Research in Chagas´Disease, realizado en
Caxambú, MG, Brazil. (02-05/09/1999).
46. Efects of fungal sphingolipid biosynthetic pathway inhibitors over trypanosoma cruzi in
axenic culture. Paula-Lima A.C., Santiago A.P., Mendonça-Previato L., Previato J.O.,
Heise N. Annals of the XXVI Annual Meeting on Basic Research in Chagas´Disease,
Caxambú, MG, Brazil. (02-05/09/1999).
47. Ação de antifúngicos na biossíntese de esfingolipídeos em Trypanossoma cruzi. PaulaLima A.C., Mendonça-Previato L., Previato J.O., Heise N. Anais da V Semana de
Microbiologia e Imunologia, Instituto de Microbiologia Professor Paulo de Góes, Rio
de Janeiro, RJ, Brazil (27/09 – 01/10/1999)
48. Partial purification of O-alfa-N-acetilglucosaminyl transferase of trypanosoma cruzy ystrain. Dias W.B., Paula-Lima A.C., Agrellos O.A., Solla-Pena M., Heise N.,
Mendonça-Previato L., Previato J.O. Annals of the XXVII Annual Meeting of the
Brazilian Society of Biochemistry and Molecular Biology, Caxambu, MG, Brazil. (1620/05/1999).
49. Contributions of eletrostatic interactions to the stability of amyloid fibrils formed by
amylin and beta-amyloid. Lopes, D.H., Louzada, P.R., Paula-Lima A.C., De Felice
F.G., Ferreira S.T. Annals of the XXX Annual Meeting of the Brazilian Society of
Biochemistry and Molecular Biology, Caxambú, MG, Brazil. (19-22/05/2001).
50. Role of glutamatergic and gabaergic transmission on beta-amyloid neurotoxicity.
Possible implications for alzheimers disease therapeutics. Louzada P.R., Paula-Lima
A.C., Mendonça-Silva D.L., Noel F.N., de Mello F.G., Ferreira S.T. Arquivos de
Neuro-psiquiatria - Jornal Oficial da Academia Brasileira de Neurologia. III Reunião
de Pesquisadores em Doença de Alzheimer e Desordens Relacionadas, Rio de Janeiro,
RJ, Brazil (24/11/2001).
51. Role of glutamatergic neurotransmission on amyloid beta 1-42 aggregation and
neurotoxicity in embryonic avian retina. Paula-Lima A.C., Louzada P.R., MendonçaSilva D.L., Noel F.F., de Mello F.G., Ferreira S.T. Annals of the XXX Annual Meeting
of the Brazilian Society of Biochemistry and Molecular Biology, Caxambú, MG, Brazil.
(19-22/05/2001).
52. Inhibitors of sphingolipid biosynthesis and their effects on trypanosoma cruzi growth in
an axenic culture. Paula-Lima A.C., Santiago A.P., Mendonça-Previato L., Previato
J.O. Annals of the XXIX Annual Meeting of the Brazilian Society of Biochemistry and
Molecular Biology realizado em Caxambú, MG, Brazil. 27 a 30/05/2000).
53. Ether lipids X ceramides in GPI anchors of trypanosomatids: first round. Heise, N.,
Paula-Lima A.C., Hart, D.T., Mendonça-Previato L. and Previato J.O. e Heise N.
Annals of the XXVI Annual Meeting on Basic Research in Chagas´Disease, Caxambú,
MG, Brazil. (02-05/09/1999).
54. Effects of fungal sphingolipid biosynthetic pathway inhibitors over trypanosoma cruzi
in axenic culture. Paula-Lima A.C., Santiago A.P., Mendonça-Previato L., Previato
J.O., Heise N. Annals of the XXVI Annual Meeting on Basic Research in
Chagas´Disease, Caxambú, MG, Brazil. (02-05/09/1999).
55. Ação de antifúngicos na biossíntese de esfingolipídeos em Trypanossoma cruzi. PaulaLima A.C., Mendonça-Previato L., Previato J.O., Heise N. Anais da V Semana de
Microbiologia e Imunologia, Instituto de Microbiologia Professor Paulo de Góes
(27/09 – 01/10/1999)
56. Partial purification of O-alfa-N-acetilglucosaminyl transferase of trypanosoma cruzy ystrain. Dias W.B., Paula-Lima A.C., Agrellos O.A., Solla-Pena M., Heise N.,
Mendonça-Previato L., Previato J.O. Annals of the XXVII Annual Meeting of the
Brazilian Society of Biochemistry and Molecular Biology, realizado em Caxambú –
Minas Gerais – Brasil. (16-20/05/1999).