Pollen storage and vitamin B stability

Pollen storage and vitamin B stability

VITAMIN B STABILITY OF DRIED BEE
POLLEN DURING STORAGE
Prof. Assoc. Ligia Bicudo de Almeida Muradian
Food and Nutrition Department -Pharmaceutical Science School
University of São Paulo, Brazil
[email protected]
Countries which has bee pollen
regulation:
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Argentina
Armenia
Bulgaria
Brazil
China
Cuba
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Poland
Russia
Switzerland
Turkey
Uruguay
BRAZIL
Production of 200 ton/year
Bee pollen production
Collect...............
Freeze
Dehydration..............................................
Cleaning
Package.......
Bee pollen collect
Vitamins studied:
(water-soluble vitamins of the B complex)
Vitamin B1 (Thiamin)
deficiency = beriberi
(neurological and cardiovascular disease)
Vitamin B2 (Riboflavin)
deficiency = ariboflavinosis
(include cracked and red lips,
inflammation of the lining of mouth)
Vitamins studied:
(water-soluble vitamins of the B complex)
Vitamin B3 (PP or niacin)
deficiency = pellagra
(diarrhea, dermatitis and dementia)
Nicotinic acid
Nicotinamide
Vitamin B6 (Piridoxine)
3 isomers
Piridoxine
Piridoxal
Piridoxamine
Material and Methods
Material
07 Batches of bee pollen from Sao Paulo, Brazil were
collected using 15 bee hives (Apis mellifera) and were kept
at room temperature (with and without light exposure) an in
freezer
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Methods
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Simultaneous extraction (Moreschi, 2006;
Presoto and Almeida-Muradian 2008)
HPLC:
Vitamin B1 (pre-column reaction): Mobile phase: phosfate buffer pH 7.2
and dimethylformamide; Column: C18; fluorescence detection (Ex 368 nm;
Em 440 nm)
Vitamin B2: Mobile phase: phosfate buffer pH 7.2 and dimethylformamide;
Column: C18; fluorescence detection (Ex 450 nm; Em 530 nm)
Vitamin PP (post-column reaction): phosfate buffer with hidrogen
peroxide and copper sulphate, cobre; Column: C18 ; fluorescence detection
(Ex 322nm;
Em 380 nm)
Vitamina B6: phosfate buffer with pH 2.5 with ion par and acetonitrile:
Column: C18; fluorescence detection (Ex 296nm; Em 390 nm)
Chromatograms
Standards
B1
B2
PP
Samples
Chromatograms
B6
Standard
Sample
Results
Vitamin B1
mg/100g
0.80
0.70
Stable
0.60
Light
0.50
inicial
Initial
4 meses
8 meses
12 meses
Months
Light
Luz
Dark
Escuro
Freezer
Initial
concentration
Teor
inicial
Results
mg/100g
2,7
Vitamin
Vitamina B2
2,2
1,7
a
ab
a
a
a
a
a
a
a
1,2
inicial
Initial
4 meses
Light
Luz
Escuro
Dark
8 meses
Freezer
months
12 meses
Initial
concentration
Teor
inicial
28% lost
Still vitamin source
Results
Vitamin
Vitamina PP
mg/100g
13
11
9
7
a
5
a
a
3
inicial
Initial
4 meses
Luz
Light
Escuro
Dark
8 meses
Freezer
12
12meses
months
Initial
Teorconcentration
inicial
60% lost
Results
Vitamina B6
Vitamin
mg/100g
0,35
0,1
a
-0,15
a
a
-0,4
Initial
inicial
4 meses
Luz
Light
Escuro
Dark
8 meses
Freezer
12months
meses
12
Teor inicial
Initial concentration
total lost
Conclusion
• After one year the vitamins were:
• B1 Æ remain
• B2 Æ 28% lost
• PP Æ 60% lost
• B6 Æ 100% lost
• The vitamin loose depends more on the
time of storage than the conditions of
storage.
Thank you all...
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Vanilda Arruda (PhD student, USP)
Aline Pereira (Undergraduate student, USP)
Ortrud Monika Barth (PhD, FIOCRUZ)
Alex Freitas (MS student, FIOCRUZ)
Fabricia Soriane (PRONATU)