WORK PACKAGE 1

International Research Staff Exchange
Scheme (IRSES)
Better Understanding of Bugs for
Improved Environment – BUGSIE
(PIRSES-GA-2012-317981)
Lincoln University March 5-7 2014
PARTICIPANTS
ü  EU Member States
ü  Countries with an S&T
agreement with the EC
Coordinating Organisation
University of Palermo
Dep of Agricultural and Forest Sciences
Participating Organisation
Institut National de la Recherche Agronomique
Participating Organisation
Julius-Maximilians Universität Würzburg
Department of Botany II
Lincoln University
The Bio-Protection Research Centre
New Zealand Institute for Plant and
Food Research Ltd
PARTICIPANTS
Lincoln University
Michael Rostas (Experienced researcher)
Stephen Wratten (Experienced researcher)
Stephane Boyer (Experienced researcher)
Maya Raad (Early stage researcher)
New Zealand Institute for Plant and Food
Research Ltd
Kye Chung Park (Experienced researcher)
PARTICIPANTS
Stefano Colazza (Coordinator)
Ezio Peri (Experienced researcher)
Antonino Cusumano (Experienced researcher)
Maria Cristina Foti (Early stage researcher)
Takoua Slimani (Early stage researcher)
Eric Wajnberg (Experienced researcher)
Anne-Violette Lavoir (Experienced researcher)
Michael Riedel (Experienced researcher)
PARTICIPANTS BUGSIE MEETING
LINCOLN UNIVERSITY
FLTR: Stephen Wratten, Maria Cristina Foti, Ezio Peri, Stephane Boyer, Kye
Chung Park, Maya Raad, Michael Rostas, Stefano Colazza, Michael Riedel
MEETING AGENDA
Wednesday,)5th)March)
10:009
Welcome!
10:30!
reception!
10:309
Tour!of!the!
12:00!
BPRC!
Lunch!
13:309
Progress!
17:00!
reports!
Thursday,)6th)March)
09:009
Progress!
12:00!
reports!and!
discussion!
!
19:30!
!
13:309
17:00!
Friday,)7th)March)
09:009
Progress!
12:00!
reports!and!
discussion!
Lunch!
Lunch!
Lab!visit!Plant! Tour!of!Biotron!
and!Food!
Research!
Discussion!on!
specific!tasks!
Dinner!
!
INTERNATIONAL RESEARCH STAFF EXCHANGE
SCHEME (IRSES) - MARIE CURIE ACTIONS
Full Title
Better Understanding of
Bugs for Improved
Environment
Acronym
BUGSIE
Proposal Number
317981
Grant Agreement Number
PIRSES-GA-2012
Duration of the project
months 36
Project start date
January 2013
Project end date
December 2015
ESTABLISHMENT OF THE MANAGEMENT
STRUCTURE
Management Board
Project Office
Colazza
Riedel
Wajnberg
Rostas
Project coordinator = Colazza
Project assistant management = Peri
WP1
WP2
WP3
WP4
WP5
Riedel
Wratten
Rostas
Cusumano
Colazza
General meeting BUGSIE partners
WORK PACKAGE
WORK PACKAGE 1 (Riedel)
Work package number
Work package title
1
Start date or starting
1-24
event:
Geographical variations T. basalis foraging behavior
Beneficiary/Partner
UNIPA – UNIWUE – INRA – LU – PFR
Organization short
names
Objectives
The object of this WP is to evaluate geographical variations in T. basalis foraging
behavior in response to synomones and kairomones
Description of works
Task 1.1: amended.
Task 1.2: Analyse cuticular hydrocarbons from different N. viridula populations.
Task 1.3: Evaluate T. basalis sensory response to cuticular hydrocarbons (contact
kairomones)
Task 1.4: Evaluate T. basalis behavioural response to cuticular hydrocarbons (contact
kairomones)
Task 1.5: Establish genetic differences in T. basalis populations using microsatellite
analysis.
Deliverables
D1.1: Manuscript submitted to peer-reviewed journal and/or growers magazines on
factors that could affect T. basalis host location
D1.2: Find T. basalis strain that better responds to synomones and kairomones
WORK PACKAGE 1 (Riedel)
Presentation of work
done so far
See Michael Rostas and Michael
Riedel presentations
Task 1.2 first step accomplished
Outline and discussion of
planned work
Tasks 1.2-1.5 methodology
discussed
Discussion of critical
issues and how work
aligns with BUGSIE
schedule
Task 1.1. replaced by GC-EAD with
cuticular hydrocarbons extracts
with T. basalis
Task 1.5 development based on
budget availability
Discussion whether
amendments to schedule
are necessary
Extend tasks 1.3 & 1.5 to 36
months, allocation of other ESR to
work on 1.3
WORK PACKAGE 2 (Wratten)
Work package number
Work package title
2
Start date or starting
event:
Conservation Biological Control
12-36
Beneficiary/Partner
UNIPA - INRA - LU - PFR
Organization short
names
Objectives
Develop habitat manipulation strategies to reduce crop colonization rates of Nezara
viridula and improve efficiency of T. basalis
Description of works
Task 2.1 Amended
Task 2.2 Amended
Task 2.3 Analyses of searching behaviour of T. basalis in response to floral resources.
Task 2.4 Evaluation of added field floral resources to increase parasitism rates.
Deliverables
D2.1: Manuscript submitted to peer-reviewed journal
D2.2: Effective combination of floral resources for use in conservation biocontrol
WORK PACKAGE 2 (Wratten)
Presentation of work
done so far
See Cristina’s presentation
Outline and discussion of
planned work
Tasks 2.2., 2.3, 2.4 methodology
discussed
Discussion of critical
issues and how work
aligns with BUGSIE
schedule
Task 2.1 Evaluation of trap
cropping in EU and compare with
published results in NZ.
Replaced by: GC-EAD with floral
odorants with T. basalis
Task 2.2 Investigating longevity of
T. basalis in response to floral
nectar = Reintroduced
Discussion whether
amendments to schedule
are necessary
Not necessary
WORK PACKAGE 3 (Rostas)
Work package number
Work package title
3
Start date or starting
12-36
event:
T. basalis behaviour in a multitrophic context
Beneficiary/Partner
UNIPA - LU - PFR - INRA
Organization short
names
Objectives
Investigate the performance of Nezara viridula and the foraging behaviour of Trissolcus
basalis on plants that are colonized by endophytic Beauveria bassiana and by a non host
pest, Sitona lineatus.
Description of works
Task 3.1 Measure performance and behaviour of Nezara viridula on fungus-infected
plants or on plant damaged by non-host pest.
Task 3.2 Measure behavioural responses of egg parasitoids on fungus-infected plants
that carry host eggs or on plant damaged by non-host pest.
Task 3.3 Analyse herbivore-induced synomones from fungus-infected plants or on plant
damaged by non-host pest..
Deliverables
D3.1: Manuscript submitted to peer-reviewed journal on factors that could affect T.
basalis host location
WORK PACKAGE 3 (Rostas)
Presentation of work
done so far
See Maya’s presentation
Outline and discussion of
planned work
Work is on track
Discussion of critical
issues and how work
aligns with BUGSIE
schedule
Critical issues such as provision of
plant pathology lab equipment
were discussed
Discussion whether
amendments to schedule
are necessary
Not necessary at this stage but
feasibility of Task 3.1 will depend
on time constraints
WORK PACKAGE 4 (Cusumano)
Work package number
Work package title
4
Start date or starting
event:
Intraguild interactions in egg parasitoids
1-24
Beneficiary/Partner
UNIPA – INRA - LU
Organization short
names
Objectives
Exploring conflict/synergies in controlling N. viridula population with one or more egg
parasitoid specie.
Description of works
Task 4.1: The role of chemical communication in contest behaviour.
Task 4.2: The importance of asymmetries in the value of resources (host-quality) to the
contestant females and of host quality to the evolution of the host-parasitoid association.
Task 4.3: The parallels and differences between laboratory and field situations and the
implications of these for the application of behavioural studies to biological control.
Deliverables
D4.1: Identification of chemical cues playing a role in contest outcome
D4.2: Characterization of host quality and its influence on contest behavior
D4.3: Improved understanding of the role of contest behaviour in the field and parasitoid
strains evaluated in terms of efficiency of host exploitation under agricultural greenhouse
conditions.
WORK PACKAGE 4 (Cusumano)
Presentation of work done so See Ezio’s presentation
far
Task 4.3 Manuscript submitted
Outline and discussion of
planned work
Work is on track
Discussion of critical issues
and how work aligns with
BUGSIE schedule
Task 4.2: The importance of
asymmetries in the value of resources
(host-quality) to the contestant
females and of host quality to the
evolution of the host-parasitoid
association. Replaced by: Physiological
suppression mediated by the
ovipositing O. telenomicida
Discussion whether
No amendments are necessary
amendments to schedule are
necessary
WORK PACKAGE 5 (Colazza)
Work package number
Work package title
5
Start date or starting
event:
Project Networking/Dissemination
1-36
Beneficiary/Partner
UNIPA – UNIWUE – INRA – LU – PFR
Organization short
names
Objectives
The objective of this work package is to manage the effective dissemination of knowledge
between the Network Partners and international stakeholders.
Description of works
Task 5.1: Consortium Network website for project participants.
Task 5.2: Collation and dissemination of work packages to and from partners.
Task 5.3: BUGSIE Workshops
Deliverables
D5.1: BUGSIE Network website.
D5.2: Annual summary of Network work packages supplied to participants.
D5.3: Abstracts from Network Workshop published in conference proceedings.
D 5.4: Dissemination to stakeholders.
WORK PACKAGE 5 (Colazza)
Presentation of work
done so far
- SHARE DOCUMENTS: the documents
are shared among the partners using a
folder named ‘BUGSIE’ located on a
web cloud (Drop Box)
-  WEBSITE:
http://pirsesbugsie.wordpress.com
-  MANUSCRIPT: see WP4
Outline and discussion
of planned work
Work is on track
Discussion of critical
issues and how work
aligns with BUGSIE
schedule
Stakeholders identified in ‘BIOFORCE –
NZ’, ‘HEINZ-WATTIES – NZ’ and
‘CEDENCO – NZ’
Discussion whether
amendments to
schedule are necessary
Not necessary
SECONDMENT STRATEGY
Revised during the 2nd meeting
PARTICIPANT
NUMBER
ORIGIN
PARTNER
ORIGIN
COUNTRY
HOST
PARTNER
HOST
COUNTRY
SECONDED
TYPE
DURATION
(MONTHS)
STARTING
MONTH
1
UNIPA
IT
LU
NZ
Stefano
MS
1
1
UNIPA
IT
LU
NZ
Maria Cristina
ESR
12
15
9
UNIPA
IT
LU
NZ
Takoua
ESR
8
21
1
UNIPA
IT
LU
NZ
Ezio
ER
1
15
1
UNIPA
IT
PFR
NZ
Maria Cristina
ESR
1
35
1
UNIPA
IT
PFR
NZ
Antonio
ER
1
35
1
UNIPA
IT
PFR
NZ
Stefano
MS
1
35
1
UNIPA
IT
PFR
NZ
Ezio
ER
1
35
2
UNIWUE
D
LU
NZ
Michael
ER
1
15
2
UNIWUE
D
PFR
NZ
Michael
ER
2
34
3
INRA
FR
LU
NZ
Eric
ER
1
15
3
INRA
FR
PFR
NZ
Eric
ER
2
34
3
INRA
FR
PFR
NZ
Anne-Violette
ER
2
34
4
LU
NZ
UNIPA
IT
Michael
ER
1
5
4
LU
NZ
UNIPA
IT
Stephane
ER
1
5
4
LU
NZ
UNIWUE
IT
Steve
ER
1
29
4
LU
NZ
UNIPA
IT
Maya
ESR
4
18
3
LU
NZ
UNIWUE
D
Michael
ER
1
29
4
LU
NZ
UNIWUE
D
Michael
ER
2
19
5
PFR
NZ
UNIWUE
IT
Kye Chung
ER
1
29
(Lincoln University)
1
2
1
2
3
4
5
6
3
1
2
3
4
5
6
7
8
9
10 11 12
7
8
9
10 11 12
1
2
3
4
5
6
1
2
3
4
5
6
7
8
9
10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36
7
8
9
10 11 12
THE TABLE OF TRANSFERS OF FUNDS
BETWEEN PARTNERS
pre-fin of 39.270 Eur has been paid on the 23/03/2013 to the coordinator
number of months found/months 2nd payment (40%) total contribuFon 54600.00 32760.00 21840.00 54600.00 2730.00 30030.00 4200.00 10500.00 525.00 5775.00 FROM COUNTRY TO COUNTRY UNIPA Italy LU New Zealand 2 2100.00 4200.00 UNIPA Italy LU New Zealand 8 2100.00 16800.00 UNIPA Italy LU New Zealand 2 2100.00 4200.00 UNIPA Italy PFR New Zealand 8 2100.00 16800.00 UNIPA Italy PFR New Zealand 3 2100.00 6300.00 UNIPA Italy PFR New Zealand 3 2100.00 6300.00 total INRA France LU New Zealand 1 2100.00 2100.00 INRA France PFR New Zealand 2 2100.00 4200.00 INRA France PFR New Zealand 2 2100.00 4200.00 total 5 26 ContrbuFon to the effecFve 1st Guarantee payment Fund EU (5%) 1st payment (60%) total euro 10500.00 6300.00 UNIWE Germany LU New Zealand 1 2100.00 2100.00 UNIWE Germany PFR New Zealand 1 2100.00 2100.00 UNIWE Germany PFR New Zealand 1 2100.00 2100.00 total 3 6300.00 3780.00 2520.00 6300.00 315.00 3465.00 34 71400.00 42840.00 28560.00 71400.00 3570.00 39270.00 TOTAL GENERAL GRANT AGREEMENT REPORTING
•  Only two reports in the whole project, the first after 21
months
•  simple but critical:
– pay extreme attention to complete everything in the online system (be prepared for);
– ERA is usually late on subsequent payments;
ORGANIZATION OF THE 2nd GENERAL
MEETING.
•  Workshop #1. University of Palermo, May 6-8 2013 Italy: This workshop took
place at the beginning of the project and set the scene for the research visits
that follow. Partners present summaries of their previous research and
discussions and brainstorming sessions followed each talk. Work packages
were adjusted accordingly. Folios (hard copy and electronic) of the PowerPoint
presentations and is prepared and shared by the web cloud.
•  Workshop #2. planned for March 2014 in Lincoln University in New Zealand.
This workshop will take place midway through the programme. Updates of the
work packages will be provided to the partners and where necessary fine-tuning
of the work packages may take place.
•  Workshop #3. planned for May 2015 in INRA (Sophia Antipolis) in France/
Wuerzburg in Germany. Updates of the work packages will be provided to the
partners and where necessary fine-tuning of the work packages may take place.
•  Workshop #4. planned November/December 2015 in Lincoln University in New
Zealand. This workshop will take place at the end of the project and will allow
partners to look back on the research carried out in the different work packages.
Priorities for future work will be discussed and plans for future research
collaborations confirmed.